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| Collares,E.F.; Vinagre,A.M.; Collares-Buzato,C.B.. |
| Atropine (AT) and dipyrone (Dp) induce a delay of gastric emptying (GE) of liquids in rats by inhibiting muscarinic receptors and activating β2-adrenergic receptors, respectively. The objective of the present study was to determine the effects of pretreatment with AT and Dp, given alone or in combination, on the effect of hypoglycemia in the liquid GE in rats. Male Wistar adult rats (280-310 g) were pretreated intravenously with AT, Dp, AT plus Dp or their vehicle and then treated 30 min later with iv insulin or its vehicle (n=8-10 animals/group). Thirty min after treatment, GE was evaluated by determining, in awake rats, the percent gastric retention (%GR) of a saline meal labeled with phenol red administered by gavage. The results indicated that insulin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hypoglycemia; Gastric emptying; Dipyrone; Atropine; Muscarinic receptors; Adrenergic receptors. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000610 |
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| Santos,Roberta Tancredi Francesco dos; Silva,Marcelo Florencio Passos; Porto,Rafael Marques; Lebrun,Ivo; Gonçalves,Luís Roberto de Camargo; Batista,Isabel de Fátima Correia; Sandoval,Maria Regina Lopes; Abdalla,Fernando Maurício Francis. |
| Abstract Background: Here, we described the presence of a neurotoxin with phospholipase A2 activity isolated from Micrurus lemniscatus venom (Mlx-8) with affinity for muscarinic acetylcholine receptors (mAChRs). Methods: The purification, molecular mass determination, partial amino acid sequencing, phospholipase A2 activity determination, inhibition of the binding of the selective muscarinic ligand [3H]QNB and inhibition of the total [3H]inositol phosphate accumulation in rat hippocampus of the Mlx-8 were determined. Results: Thirty-one fractions were collected from HPLC chromatography, and the Mlx-8 toxin was used in this work. The molecular mass of Mlx-8 is 13.628 Da. Edman degradation yielded the following sequence:... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Muscarinic receptors; Hippocampus; Micrurus lemniscatus; Inositol phosphate; Phopholipase A2. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100301 |
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| Sanches,G.; Ventura,A.L.M.. |
| In the present study, we report that low concentrations of the glutamate ionotropic agonist kainate decreased the turnover of [3H]-phosphoinositides ([3H]-InsPs) induced by muscarinic receptors in the chick embryonic retina. When 100 µM carbachol was used, the estimated IC50 value for kainate was 0.2 µM and the maximal inhibition of ~50% was obtained with 1 µM or higher concentrations of the glutamatergic agonist. Our data also show that veratridine, a neurotoxin that increases the permeability of voltage-sensitive sodium channels, had no effect on [3H]-InsPs levels of the embryonic retina. However, 50 µM veratridine, but not 50 mM KCl, inhibited ~65% of the retinal response to carbachol. While carbachol increased [3H]-InsPs levels from 241.2 ± 38.0 to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Muscarinic receptors; Phosphoinositides; Kainate; Sodium channels; Retina. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700009 |
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